Genomic DNA Extraction for Molecular Identification of Endophytic Fungi: An Easy and Efficient Protocol
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چکیده
منابع مشابه
A simple and rapid DNA extraction protocol for filamentous fungi efficient for molecular studies
A simple and rapid protocol for extracting high-quality DNA from filamentous fungi was studied. The method involved disruption of fungal cells by employing glass bead method, followed by inactivation of proteins using CTAB/proteinase K. The DNA yield from fungal isolates varied from 310-1879 μg g dry mycelium and a clear intact DNA band was observed upon agarose gel electrophoresis. Absorbency ...
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High-quality DNA extraction plays an important role to make sharp bands in the gel electrophoresis and also produces clean chromatograms. Usually, DNA extract is delivered using the modified CTAB method but this method cannot obtain high-quality DNA for molecular analysis from old dried leaves of Bellevalia due to having different chemical compounds which inhibit to obtain a clear DNA extractio...
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چکیده ندارد.
a rapid and reproducible genomic dna extraction protocol for sequence-based identification of archaea, bacteria, cyanobacteria, diatoms, fungi, and green algae
background : sequence-based identification of various microorganisms including archaea, bacteria, cyanobacteria, diatoms, fungi, and green algae necessitates an efficient and reproducible genome extraction procedure though which a pure template dna is yielded and it can be used in polymerase chain reactions (pcr). considering the fact that dna extraction from these microorganisms is time consu...
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DNA isolation from some fungal organisms is difficult because they have cell walls or capsules that are relatively unsusceptible to lysis. Beginning with a yeast Saccharomyces cerevisiae genomic DNA isolation method, we developed a 30-min DNA isolation protocol for filamentous fungi by combining cell wall digestion with cell disruption by glass beads. High-quality DNA was isolated with good yie...
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ژورنال
عنوان ژورنال: Biosciences, Biotechnology Research Asia
سال: 2017
ISSN: 0973-1245,2456-2602
DOI: 10.13005/bbra/2492